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Du Bois's health declined during the two years he was in Ghana, and he died on August 27, 1963, in the capital of Accra at the age of 95.
Du Bois was given a state funeral on August 29–30, 1963, at Nkrumah's request, and buried beside the western wall of Christiansborg Castle (now Osu Castle), then the seat of government in Accra.